Braak stage 1, 100% of pigmented neurons left
parkinsonconnecting

Gene record

IFT122

intraflagellar transport 122

translation score 0.55, read 2026-10-18; 1 sealed predictions, 0 held; 1 receipts.

Six readers

readerfoundscore
genetics
Open Targets		genetic association 0.38 (overall 0.23)0.38
cell type
Human Protein Atlas		expressed broadly, no clear home in brain cells0.40
chemistry
Open Targets		hard to drug0.00
trials
ClinicalTrials.gov		no Parkinson's trials on record: an open field1.00
literature
PubMed		no papers tie it to Parkinson's yet1.00
pathways
Reactome		2 pathways; mostly growth signalling0.60
proposal for IFT122, translation score 0.55

Knock down and overexpress IFT122 in a dopamine neuron, astrocyte and microglia tri-culture; measure phospho-ERK and phospho-AKT response to GDNF and dopamine neuron survival after α-synuclein fibril challenge.

Cell type
a dopamine neuron, astrocyte and microglia tri-culture
Direction
Both arms: public data does not say which way helps.
Molecular readout
phospho-ERK and phospho-AKT response to GDNF
Functional readout
dopamine neuron survival after α-synuclein fibril challenge
Rule-out
If neither arm moves phospho-ERK by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
Lab request
CRISPRi knockdown and lentiviral overexpression of IFT122 in a tri-culture of iPSC dopamine neurons, astrocytes and microglia; n = 3 differentiations; readouts: phospho-ERK and phospho-AKT response to GDNF; dopamine neuron survival after α-synuclein fibril challenge. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.

Sealed predictions

  1. IFT122 in dopamine neurons, braak-0.43 (p 0.243) → +0.06 (p 0.873)receipt 1835

Swarm cases

receipt 1835: IFT122 in dopamine neurons, braak

power
full sample -0.22 (p 0.379, n 18), 95% CI -0.62 to +0.27. the interval is wide: not enough donors to tell.
confounder
adjusted for sex: -0.21 (p 0.393, n 18). does not hold up.
composition
dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: -0.39 (p 0.114, n 18). could be the mix.
skeptic
leave-one-out range -0.35 to -0.12, sign flips 0; without severely affected donors -0.22 (p 0.379, n 18). fragile.
cell type
no other cell group does better.
axis
no measure beats "tracks Braak Lewy-body stage" for IFT122 in dopamine neurons.

case closed: nothing better to follow up.

Receipts

loading the receipts