Six readers
| reader | found | score |
|---|---|---|
| genetics Open Targets | no genetic evidence; overall 0.01 from other evidence | 0.00 |
| cell type Human Protein Atlas | cell type enhanced, including oligodendrocytes; strongest in pituicytes/fscs | 0.75 |
| chemistry Open Targets | a small-molecule pocket: collagenase clostridium histolyticum, ocriplasmin | 0.60 |
| trials ClinicalTrials.gov | 1 Parkinson's trial, 0 stopped | 1.00 |
| literature PubMed | no papers tie it to Parkinson's yet | 1.00 |
| pathways Reactome | 15 pathways; mostly protein handling | 0.90 |
Knock down and overexpress COL4A5 in oligodendrocytes; measure proteasome activity and the load of ubiquitinated protein and α-synuclein uptake and myelin basic protein output under oxidative stress.
- Cell type
- oligodendrocytes
- Direction
- Both arms: public data does not say which way helps.
- Molecular readout
- proteasome activity and the load of ubiquitinated protein
- Functional readout
- α-synuclein uptake and myelin basic protein output under oxidative stress
- Rule-out
- If neither arm moves proteasome activity by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
- Lab request
- CRISPRi knockdown and lentiviral overexpression of COL4A5 in iPSC-derived oligodendrocytes; n = 3 differentiations; readouts: proteasome activity and the load of ubiquitinated protein; α-synuclein uptake and myelin basic protein output under oxidative stress. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.
Sealed predictions
- COL4A5 in dopamine neurons, braak+0.23 (p 0.551) → -0.11 (p 0.773)receipt 10603
- COL4A5 in oligodendrocytes, braak-0.27 (p 0.485) → +0.74 (p 0.023)receipt 10602
Swarm cases
receipt 10603: COL4A5 in dopamine neurons, braak
- power
- full sample +0.11 (p 0.658, n 18), 95% CI -0.37 to +0.55. the interval is wide: not enough donors to tell.
- confounder
- adjusted for sex: +0.16 (p 0.521, n 18). does not hold up.
- composition
- dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: +0.29 (p 0.246, n 18). could be the mix.
- skeptic
- leave-one-out range +0.02 to +0.28, sign flips 0; without severely affected donors +0.11 (p 0.658, n 18). fragile.
- cell type
- no other cell group does better.
- axis
- no measure beats "tracks Braak Lewy-body stage" for COL4A5 in dopamine neurons.
case closed: nothing better to follow up.
receipt 10602: COL4A5 in oligodendrocytes, braak
- power
- full sample +0.27 (p 0.285, n 18), 95% CI -0.23 to +0.65. the interval is wide: not enough donors to tell.
- confounder
- adjusted for sex: +0.38 (p 0.123, n 18). does not hold up.
- composition
- oligodendrocytes share of nuclei vs axis +0.03 (p 0.915); controlling for it: +0.29 (p 0.239, n 18). could be the mix.
- skeptic
- leave-one-out range +0.17 to +0.40, sign flips 0; without severely affected donors +0.27 (p 0.285, n 18). fragile.
- cell type
- no other cell group does better.
- axis
- no measure beats "tracks Braak Lewy-body stage" for COL4A5 in oligodendrocytes.
case closed: nothing better to follow up.
Receipts
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