Six readers
| reader | found | score |
|---|---|---|
| genetics Open Targets | genetic association 0.53 (overall 0.33) | 0.53 |
| cell type Human Protein Atlas | cell type enhanced, including brain excitatory neurons; strongest in cone photoreceptor cells | 0.75 |
| chemistry Open Targets | a small-molecule pocket | 0.60 |
| trials ClinicalTrials.gov | no Parkinson's trials on record: an open field | 1.00 |
| literature PubMed | 1 Parkinson's papers of 152 | 0.91 |
| pathways Reactome | 15 pathways; mostly immune signalling | 0.90 |
Knock down and overexpress ADCY1 in dopamine neurons; measure IL-1β and TNF release and surface HLA-DR and survival of TH-positive neurons and phospho-α-synuclein (pS129) inclusions after seeding with α-synuclein fibrils.
- Cell type
- dopamine neurons
- Direction
- Both arms: public data does not say which way helps.
- Molecular readout
- IL-1β and TNF release and surface HLA-DR
- Functional readout
- survival of TH-positive neurons and phospho-α-synuclein (pS129) inclusions after seeding with α-synuclein fibrils
- Rule-out
- If neither arm moves IL-1β by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
- Lab request
- CRISPRi knockdown and lentiviral overexpression of ADCY1 in iPSC-derived midbrain dopamine neurons (FOXA2+, LMX1A+, TH+); n = 3 differentiations; readouts: IL-1β and TNF release and surface HLA-DR; survival of TH-positive neurons and phospho-α-synuclein (pS129) inclusions after seeding with α-synuclein fibrils. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.
Sealed predictions
- ADCY1 in dopamine neurons, braak-0.45 (p 0.219) → -0.49 (p 0.177)receipt 1006
Swarm cases
receipt 1006: ADCY1 in dopamine neurons, braak
- power
- full sample -0.40 (p 0.097, n 18), 95% CI -0.73 to +0.08. the interval is wide: not enough donors to tell.
- confounder
- adjusted for sex: -0.40 (p 0.097, n 18). does not hold up.
- composition
- dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: -0.64 (p 0.0039, n 18). not the mix.
- skeptic
- leave-one-out range -0.53 to -0.33, sign flips 0; without severely affected donors -0.40 (p 0.097, n 18). robust.
- cell type
- no other cell group does better.
- axis
- no measure beats "tracks Braak Lewy-body stage" for ADCY1 in dopamine neurons.
case closed: nothing better to follow up.
Receipts
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